Kit components: Wash Buffer (Reagent A) and the "all in one" goat anti-rabbit HRP-conjugated secondary antibody in advanced blocking solution (Reagent B).Required: primary antibody of your choice and
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The IsiA protein standard can be used in combination with anti-IsiA antibodies to quantitate IsiA from a range of cyanobacteria. Global antibodies are raised against highly conserved amino acid sequen
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Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot
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