GST monoclonal antibody, clone 3D4
产品名称： GST monoclonal antibody, clone 3D4
英文名称： GST monoclonal antibody, clone 3D4
产品编号： MAB1996
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against GST.
- Immunogen:
- Glutathione-S-Transferase (GST) from Schistosoma japonicum.
- Host:
- Mouse
- Reactivity:
- S. japonicum
- Form:
- Liquid
- Isotype:
- IgG1, kappa
- Storage Buffer:
- In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against GST-tag.
- Recommend Usage:
- ELISA (1:20000-1:100000)
Western Blot (1:1000-1:10000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.
Smith DB, Davern KM, Board PG, Tiu WU, Garcia EG, Mitchell GF.Proc Natl Acad Sci U S A. 1986 Nov;83(22):8703-7.
- Applications
- Western Blot
- Western blot using GST monoclonal antibody, clone 3D4 (Cat # MAB1996) to detect purified GST recombinant protein (left) and in a lysate of HeLa cells expressing recombinant GST protein (right).
Approximately 0.1 ug of protein was separated by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/mL for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of IRDye™800 conjugated Gt-a-Mouse IgG [H&L] MX.